Mutation of arginine 98, which serves as a substrate-recognition site of D-amino acid aminotransferase, can be partly compensated for by mutation of tyrosine 88 to an arginyl residue.

Abstract excerpt

D-Amino acid aminotransferase is the only aminotransferase that catalyzes the transamination of D-amino acids. We studied the role of the binding site for the alpha-carboxyl group of substrates, which is presumably crucial for the unique stereospecificity of the enzyme. The site-directed mutagene...

Topics

• Arginine
• Binding Sites
• Hydrogen-Ion Concentration
• Ketoglutaric Acids
• Kinetics
• Mutagenesis, Site-Directed
• Mutation
• Protein Conformation
• Substrate Specificity
• Transaminases
• Tyrosine