Article
Gating induces a conformational change in the outer vestibule of ENaC.
The Journal of general physiology - 1 Dec 2000
Snyder P M, Bucher D B, Olson D R
Abstract excerpt
The epithelial Na(+) channel (ENaC) is comprised of three homologous subunits (alpha, beta, and gamma). The channel forms the pathway for Na(+) absorption in the kidney, and mutations cause disorders of Na(+) homeostasis. However, little is known about the mechanisms that control the gating of ENaC. We investigated the gating mechanism by introducing bulky side chains at a position adjacent to the extracellular...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Animals
- Electric Conductivity
- Epithelial Sodium Channels
- Humans
- Ion Channel Gating
- Molecular Conformation
- Molecular Sequence Data
- Mutation
- Oocytes