Article
"Fluorescent timer": protein that changes color with time.
Science (New York, N.Y.) - 24 Nov 2000
Terskikh A, Fradkov A, Ermakova G, Zaraisky A, Tan P, Kajava A V, Zhao X, Lukyanov S, Matz M, Kim S, Weissman I, Siebert P
Abstract excerpt
We generated a mutant of the red fluorescent protein drFP583. The mutant (E5) changes its fluorescence from green to red over time. The rate of color conversion is independent of protein concentration and therefore can be used to trace time-dependent expression. We used in vivo labeling with E5 to measure expression from the heat shock-dependent promoter in Caenorhabditis elegans and from the Otx-2 promoter in...
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