Article
Bud6 directs sequential microtubule interactions with the bud tip and bud neck during spindle morphogenesis in Saccharomyces cerevisiae.
Molecular biology of the cell - 1 Nov 2000
Segal M, Bloom K, Reed S I
Abstract excerpt
In budding yeast, spindle polarity relies on a precise temporal program of cytoplasmic microtubule-cortex interactions throughout spindle assembly. Loss of Clb5-dependent kinase activity under conditions of attenuated Cdc28 function disrupts this program, resulting in diploid-specific lethality. Here we show that polarity loss is tolerated by haploids due to a more prominent contribution of microtubule-neck...
Topics
- CDC28 Protein Kinase, S cerevisiae
- Cell Polarity
- Cyclin B
- Cytoplasm
- Diploidy
- Fungal Proteins
- Genes, Lethal
- Green Fluorescent Proteins
- Haploidy
- Luminescent Proteins
- Microfilament Proteins