Article
Genome-wide variation of the somatic mutation frequency in transgenic plants.
The EMBO journal - 1 Sept 2000
Kovalchuk I, Kovalchuk O, Hohn B
Abstract excerpt
In order to analyse the frequency of point mutations in whole plants, several constructs containing single nonsense mutations in the beta-glucuronidase (uidA) gene were used to generate transgenic Arabidopsis thaliana plants. Upon histochemical staining of transgenic plants, sectors indicative of transgene reactivation appeared. Reversion frequencies were in the range of 10(-7)-10(-8) events per base pair,...
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