Article
Identification and characterization of a second butyrate kinase from Clostridium acetobutylicum ATCC 824.
Journal of molecular microbiology and biotechnology - 1 Jan 2000
Huang K X, Huang S, Rudolph F B, Bennett G N
Abstract excerpt
A gene encoding a new butyrate kinase isozyme (BKII) was identified from the C. acetobutylicum ATCC 824 DNA database. The enzyme was expressed in Escherichia coli, purified, and characterized. The purified enzyme exhibited a subunit molecular mass of 43 kDa by SDS-PAGE, and a native molecular mass of 80 kDa by gel filtration suggesting it functions as a dimer. In the butyryl phosphate-forming direction the...
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