Article
[Molecular analysis and prenatal diagnosis of beta-thalassemia: about our experience in central Tunisia].
Annales de biologie clinique - 1 Jan 2000
Laradi S, Haj Khelil A, Omri H, Chaieb A, Mahjoub T, Benlimam H, Amri F, Saad A, Miled A, Leturcq F, Ben Chibani J, Beldjord C
Abstract excerpt
Beta-thalassemia, by its high frequency and its heterogeneity, constitutes a real problem of health in Tunisia. Prenatal diagnosis by DNA analysis represents the only reality for couples at risk. The denaturant gradient (urea and formamide) on polyacrylamide gel electrophoresis has been performed in our laboratory, using psoralen as chemical clamps. This method is simple, reliable, safe, rapid, without...
Topics
- Codon
- Female
- Globins
- Heterozygote
- Homozygote
- Humans
- Molecular Epidemiology
- Mutation
- Polymerase Chain Reaction
- Pregnancy
- Prenatal Diagnosis