Article
In vivo targeting of a sunflower oil body protein in yeast secretory (sec) mutants.
The Plant journal : for cell and molecular biology - 1 Jul 2000
Beaudoin F, Wilkinson B M, Stirling C J, Napier J A
Abstract excerpt
A sunflower oleosin was expressed in yeast to study the in vivo insertion of the protein into the endoplasmic reticulum (ER) and subsequent transfer to lipid bodies. The oleosin cDNA was expressed in a range of yeast secretory (sec) mutants to determine the precise targeting pathway of the oleosin to the ER. Subcellular fractionation experiments indicated that the signal recognition particle (SRP) is required for...
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