Article
Role of the proline knot motif in oleosin endoplasmic reticulum topology and oil body targeting.
The Plant cell - 1 Aug 1997
Abell B M, Holbrook L A, Abenes M, Murphy D J, Hills M J, Moloney M M
Abstract excerpt
An Arabidopsis oleosin was used as a model to study oleosin topology and targeting to oil bodies. Oleosin mRNA was in vitro translated with canine microsomes in a range of truncated forms. This allowed proteinase K mapping of the membrane topology. Oleosin maintains a conformation with a membrane-integrated hydrophobic domain flanked by N- and C-terminal domains located on the outer microsome surface. This is a...
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