Article
Molecular cloning, overproduction and characterization of the Bacillus cereus IMP dehydrogenase.
Bioscience, biotechnology, and biochemistry - 1 Jun 2000
Kim S I, Miyamoto T, Honjoh K I, Hio M, Hatano S
Abstract excerpt
The gene of IMP dehydrogenase of Bacillus cereus ts-4, a temperature-sensitive mutant of B. cereus JCM 2152, was subcloned and its sequence was analyzed. A B. cereus ts-4 DNA fragment of 2,065 bp containing the entire impdh gene and flanking regions was sequenced. The fragment contained an open reading frame of 1,527 bp encoding 509 amino acids with a calculated molecular mass of 55,390 Da. The impdh sequence of...
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