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Article

A comprehensive systematic approach to identification of influenza A virus genotype using RT-PCR and RFLP.

Journal of virological methods - 1 Jul 2000

Offringa D P, Tyson-Medlock V, Ye Z, Levandowski R A

Abstract excerpt

Amplification of influenza A virus gene segments by reverse transcription-polymerase chain reaction (RT-PCR) can be combined with enzymatic digestion to reveal unique restriction fragment length polymorphisms specific for H1N1 and H3N2 subtype viruses. We have used the method to provide a rapid, specific and reproducible identification of the genotype of high-growth influenza reassortants derived from A/Puerto...

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Source

Identifiers

PMID: 10921838
DOI: 10.1016/s0166-0934(00)00180-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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