Article
Amino acid substitutions in a variant of IMP-1 metallo-beta-lactamase.
Antimicrobial agents and chemotherapy - 1 Aug 2000
Iyobe S, Kusadokoro H, Ozaki J, Matsumura N, Minami S, Haruta S, Sawai T, O'Hara K
Abstract excerpt
In the course of surveying for the carbapenem-hydrolyzing metallo-beta-lactamase gene bla(IMP) in pathogenic bacteria by the PCR method, we detected a gene encoding a variant metallo-beta-lactamase, designated IMP-3, which differed from IMP-1 by having low hydrolyzing activity for penicillins and carbapenems. PCR product direct sequencing of a 2.2-kb segment revealed that the gene bla(IMP-3) was located on a...
Topics
- Amino Acid Substitution
- Anti-Bacterial Agents
- Cloning, Molecular
- Kinetics
- Microbial Sensitivity Tests
- Mutation
- Polymerase Chain Reaction
- Pseudomonas aeruginosa
- Recombinant Fusion Proteins
- Shigella flexneri
- Terminology as Topic
- beta-Lactamases
- beta-Lactams