Article
Use of wide-bore capillaries in constant denaturant capillary electrophoresis.
Electrophoresis - 1 Dec 1996
Li X C, Thilly W G
Abstract excerpt
A serious limitation of capillary electrophoresis in separating mutant from wild-type DNA sequences is the amount of DNA which may be loaded on narrow-bore columns. Because we sought to examine mutant sequences derived from more than 10(9) human cells, we were obliged to overcome this limitation. Thus we examined the feasibility of using wide-bore capillaries of 250, 320 and 540 microns in constant denaturant...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
