Article
Spectroscopic characterization of the interaction between calmodulin-dependent protein kinase I and calmodulin.
Archives of biochemistry and biophysics - 1 Jul 2000
Gomes A V, Barnes J A, Vogel H J
Abstract excerpt
Calmodulin-dependent protein kinase I (CaM kinase I) is a member of the expanding class of protein kinases that are regulated by calmodulin (CaM). Its putative CaM-binding region is believed to occur within a 22-residue sequence (amino acids 299-320). This sequence was chemically synthesized and utilized for CaM interaction studies. Gel band shift assays and densitometry experiments with intact CaM kinase I and...
Topics
- Acrylamide
- Amino Acid Sequence
- Binding Sites
- Calcium-Calmodulin-Dependent Protein Kinase Type 1
- Calcium-Calmodulin-Dependent Protein Kinases
- Calmodulin
- Circular Dichroism
- Electrophoresis, Polyacrylamide Gel
- Methionine
- Molecular Sequence Data
- Mutation
- Peptide Fragments
- Protein Binding
- Protein Conformation
- Spectrometry, Fluorescence
- Tryptophan