Article
Targeting motifs and functional parameters governing the assembly of connexins into gap junctions.
The Biochemical journal - 1 Jul 2000
Martin P E, Steggles J, Wilson C, Ahmad S, Evans W H
Abstract excerpt
To study the assembly of gap junctions, connexin--green-fluorescent-protein (Cx--GFP) chimeras were expressed in COS-7 and HeLa cells. Cx26-- and Cx32--GFP were targeted to gap junctions where they formed functional channels that transferred Lucifer Yellow. A series of Cx32--GFP chimeras, truncated from the C-terminal cytoplasmic tail, were studied to identify amino acid sequences governing targeting from...
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