Article
Gap junction assembly: multiple connexin fluorophores identify complex trafficking pathways.
Cell communication & adhesion - 1 Jan 2001
Martin P E, Errington R J, Evans W H
Abstract excerpt
The assembly of gap junction channels was studied using mammalian cells expressing connexin (Cx) 26, 32 and 43 in which the carboxyl terminus was fused to green, yellow or cyan fluorescent proteins (GFP, YFP, CFP). Intracellular targeting of Cx32-CFP and 43-GFP to gap junctions was disrupted by brefeldin A treatment and resulted in a severe loss of gap junctional intercellular communication reflected by low...
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