Article
Chimeric swine vesicular disease viruses produced by fusion PCR: a new method for epitope mapping.
Journal of virological methods - 1 May 2000
Dekker A, Leendertse C H, van Poelwijk F, Rebel J M, Moormann R J
Abstract excerpt
A new method of epitope mapping based on chimeric swine vesicular disease (SVD) viruses produced by fusion PCR (polymerase chain reaction). Seven out of 16 neutralising and non-neutralising newly produced monoclonal antibodies (MAbs) discriminated between SVD isolate ITL/1/66 and NET/1/92. Using fusion PCR eight chimeric viruses were produced containing different supplementary pieces of the P1 region of both...
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