Dynamic localization of protein phosphatase type 1 in the mitotic cell cycle of Saccharomyces cerevisiae.

doi:10.1083/jcb.149.1.125

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Article

Dynamic localization of protein phosphatase type 1 in the mitotic cell cycle of Saccharomyces cerevisiae.

The Journal of cell biology - 3 Apr 2000

Bloecher A, Tatchell K

Abstract excerpt

Protein phosphatase type I (PP1), encoded by the single essential gene GLC7 in Saccharomyces cerevisiae, functions in diverse cellular processes. To identify in vivo subcellular location(s) where these processes take place, we used a functional green fluorescent protein (GFP)-Glc7p fusion protein. Time-lapse fluorescence microscopy revealed GFP-Glc7p localizes predominantly in the nucleus throughout the mitotic...

Topics

Actomyosin
Anaphase
Cell Cycle Proteins
Cell Division
Cell Nucleolus
Cell Nucleus
Cytoskeletal Proteins
Fluorescent Antibody Technique, Indirect
Fungal Proteins
Genes, Fungal
Mating Factor

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mitosis

Mutation

Peptides

Phosphoprotein Phosphatases

Profilins

Recombinant Fusion Proteins

Saccharomyces cerevisiae

Source

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Identifiers

PMID: 10747092
DOI: 10.1083/jcb.149.1.125
PMCID: PMC2175104

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