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Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow

21 Mar 2000

Abstract excerpt

Cultures of plastic-adherent cells from bone marrow have attracted interest because of their ability to support growth of hematopoietic stem cells, their multipotentiality for differentiation, and their possible use for cell and gene therapy. Here we found that the cells grew most rapidly when they were initially plated at low densities (1.5 or 3.0 cells/cm(2)) to generate single-cell derived colonies. The...

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PMID: 10725391
DOI: 10.1073/pnas.97.7.3213

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