Article
Isolation and characterization of human multiple myeloma cell enriched populations.
Journal of immunological methods - 21 Feb 2000
Tai Y T, Teoh G, Shima Y, Chauhan D, Treon S P, Raje N, Hideshima T, Davies F E, Anderson K C
Abstract excerpt
We developed a simple and rapid method to enrich tumor cells within bone marrow (BM) aspirates from patients with multiple myeloma (MM). Thirty patients with a median of 50% (8-85%) MM cells by morphology and 55% (6--85%) MM cells identified by CD38+CD45-cell surface phenotype were studied. BM mononuclear cells (BMMCs) were isolated by Ficoll Hypaque sedimentation and incubated with a cocktail of mouse monoclonal...
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