Article
Specific inhibition of barley alpha-amylase 2 by barley alpha-amylase/subtilisin inhibitor depends on charge interactions and can be conferred to isozyme 1 by mutation.
European journal of biochemistry - 1 Feb 2000
Rodenburg K W, Vallée F, Juge N, Aghajari N, Guo X, Haser R, Svensson B
Abstract excerpt
alpha-Amylase 2 (AMY2) and alpha-amylase/subtilisin inhibitor (BASI) from barley bind with Ki = 0.22 nM. AMY2 is a (beta/alpha)8-barrel enzyme and the segment Leu116-Phe143 in domain B (Val89-Ile152), protruding at beta-strand 3 of the (beta/alpha)8-barrel, was shown using isozyme hybrids to be crucial for the specificity of the inhibitor for AMY2. In the AMY2-BASI crystal structure [F. Vallée, A. Kadziola, Y....
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Binding Sites
- Calcium
- Enzyme Stability
- Hordeum
- Hydrogen Bonding
- Hydrogen-Ion Concentration
- Isoelectric Point
- Isoenzymes
- Models, Molecular
- Molecular Sequence Data
- Mutation