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Article

Mutational analysis of Escherichia coli topoisomerase IV. I. Selection of dominant-negative parE alleles.

The Journal of biological chemistry - 11 Feb 2000

Mossessova E, Levine C, Peng H, Nurse P, Bahng S, Marians K J

Abstract excerpt

In order to define regions of ParE, one of the two subunits of topoisomerase IV, that are involved in catalysis during topoisomerization, we developed a selection procedure to isolate dominant-negative parE alleles. Both wild-type parC and mutagenized parE were expressed from a tightly-regulated lac promoter on a moderate-copy plasmid. Mutated parE alleles were rescued from those plasmids that caused...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 10660569
DOI: 10.1074/jbc.275.6.4099

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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