Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

The establishment of telomerase-immortalized cell lines representing human chromosome instability syndromes

12 Feb 2000

Abstract excerpt

The limited life span of normal human cells represents a substantial obstacle for biochemical analysis, genetic manipulation and genetic screens. To overcome this technical barrier, immortal human cell lines are often derived from tumors or produced by transformation with viral oncogenes such as SV40 large T antigen. Cell lines produced by these approaches are invariably transformed, genomically unstable and...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cancer Research and Treatments
Share to Telomeres, Telomerase, and Senescence
Share to Virus-based gene therapy research

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Source

Identifiers

PMID: 10655550
DOI: 10.1093/hmg/9.3.403

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

The establishment of telomerase-immortalized cell lines representing human chromosome instability syndromesPMID 10655550Read ↗

Transient Expression of Human Telomerase Extends the Life Span of Normal Human FibroblastsSemantic proximity Read ↗

Haploinsufficiency of hTERT leads to telomere dysfunction and radiosensitivity in human cancer cells.Semantic proximity Read ↗

Use of exogenous hTERT to immortalize primary human cellsSemantic proximity Read ↗

Telomerase protects cancer-prone human cells from chromosomal instability and spontaneous immortalization.Semantic proximity Read ↗

Telomerase extends the lifespan of virus-transformed human cells without net telomere lengtheningSemantic proximity Read ↗

A human cell line that maintains telomeres in the absence of telomerase and of key markers of ALT.Semantic proximity Read ↗

Extension of life-span by introduction of telomerase into normal human cells.Semantic proximity Read ↗

Stepwise neoplastic transformation of a telomerase immortalized fibroblast cell line.Semantic proximity Read ↗

Expression of mutant telomerase in immortal telomerase-negative human cells results in cell cycle deregulation, nuclear and chromosomal abnormalities and rapid loss of viability.Semantic proximity Read ↗

Endogenous Telomerase Reverse Transcriptase N-Terminal Tagging Affects Human Telomerase Function at Telomeres In Vivo.Semantic proximity Read ↗

hTERT associates with human telomeres and enhances genomic stability and DNA repairSemantic proximity Read ↗

Functional genetics and experimental models of human cancer.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.