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Article

Characterization of an Azospirillum brasilense Tn5 mutant with enhanced N(2) fixation: the effect of ORF280 on nifH expression.

FEMS microbiology letters - 1 Feb 2000

Revers L F, Passaglia L M, Marchal K, Frazzon J, Blaha C G, Vanderleyden J, Schrank I S

Abstract excerpt

Disruption of an open reading frame (ORF) of 840 bp (280 amino acids; ORF280) in an Azospirillum brasilense Tn5 mutant resulted in a pleiotrophic phenotype. Besides an enhanced N(2)-fixing capacity and altered expression pattern of a nifH-gusA fusion, growth on the charged polar amino acids glutamate and arginine was severely affected. ORF280, similar to previously identified ORFs present in Bradyrhizobium...

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Identifiers

PMID: 10650197
DOI: 10.1111/j.1574-6968.2000.tb08928.x

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Source receipt: pubmed, retrieved 7 Sept 2026.

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