Isolation of a glutamate synthase (GOGAT)-negative, pleiotropically N utilization-defective mutant of Azospirillum brasilense: cloning and partial characterization of GOGAT structural gene.

doi:10.1128/jb.175.24.8024-8029.1993

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Article

Isolation of a glutamate synthase (GOGAT)-negative, pleiotropically N utilization-defective mutant of Azospirillum brasilense: cloning and partial characterization of GOGAT structural gene.

Journal of bacteriology - 1 Dec 1993

Mandal A K, Ghosh S

Abstract excerpt

An Azospirillum brasilense mutant (N12) pleiotropically defective in the assimilation of nitrogenous compounds (Asm-) was isolated and found lacking in the glutamate synthase (GOGAT-). The glt (GOGAT) locus of A. brasilense was identified by isolating a broad-host-range pLAFR1 cosmid clone from a gene library of the bacterium that rectified Asm- and GOGAT- defects (full recovery of activities of the nitrogenase,...

Topics

Amino Acid Sequence
Ammonium Chloride
Azospirillum brasilense
Base Sequence
Cloning, Molecular
Cosmids
Escherichia coli
Gene Library
Genes, Bacterial
Glutamate Synthase
Glutamates

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Glutamic Acid

Glutamine

Molecular Sequence Data

Mutation

Nitrogenase

Promoter Regions, Genetic

Restriction Mapping

Source

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Identifiers

PMID: 7902833
DOI: 10.1128/jb.175.24.8024-8029.1993
PMCID: PMC206984

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7902833-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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