Article
Mutants with enhanced nitrogenase activity in hydroponic Azospirillum brasilense-wheat associations.
Applied and environmental microbiology - 1 May 2000
Pereg Gerk L, Gilchrist K, Kennedy I R
Abstract excerpt
The effect of a mutation affecting flocculation, differentiation into cyst-like forms, and root colonization on nitrogenase expression by Azospirillum brasilense is described. The gene flcA of strain Sp7 restored these phenotypes in spontaneous mutants of both strains Sp7 and Sp245. Employing both constitutive pLA-lacZ and nifH-lacZ reporter fusions expressed in situ, the colony morphology, colonization pattern,...
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