Engineering of Azotobacter chroococcum enables potential replacement of synthetic nitrogen fertilizer and mitigation of nitrogen pollutants release under medium-fertility field conditions.

Abstract excerpt

Engineered free-living diazotrophs with enhanced ammonium excretion have long been proposed as a promising biofertilizer to replace chemical nitrogen fertilizers synthesized via the Haber-Bosch process. Deletion of nifL has been widely used as a strategy to engineer nitrogen-fixing strains with enhanced NH4+ excretion. However, the effects of nifL mutation on the global expression of genes and proteins in...

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