Article
Repressor mutant forms of the Azospirillum brasilense NtrC protein.
Applied and environmental microbiology - 1 Oct 2004
Huergo Luciano F, Assumpção Marcelo C, Souza Emanuel M, Steffens M Berenice R, Yates M Geoffrey, Chubatsu Leda S, Pedrosa Fábio O
Abstract excerpt
The Azospirillum brasilense mutant strains FP8 and FP9, after treatment with nitrosoguanidine, showed a null Nif phenotype and were unable to use nitrate as their sole nitrogen source. Sequencing of the ntrC genes revealed single nucleotide mutations in the NtrC nucleotide-binding site. The phenotypes of these strains are discussed in relation to their genotypes.
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