Article
Functional analysis of the GAF domain of NifA in Azospirillum brasilense: effects of Tyr-->Phe mutations on NifA and its interaction with GlnB.
Molecular genetics and genomics : MGG - 1 Jun 2005
Chen Sanfeng, Liu Li, Zhou Xiaoyu, Elmerich Claudine, Li Ji-Lun
Abstract excerpt
Regulation of NifA activity in Azospirillum brasilense depends on GlnB (a PII protein), and it was previously reported that the target of GlnB activity is the N-terminal domain of NifA. Furthermore, mutation of the Tyr residue at position 18 in the N-terminal domain resulted in a NifA protein that did not require GlnB for activity under nitrogen fixation conditions. We report here that a NifA double mutant in...
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