Article
Fetal calf serum-free generation of functionally active murine dendritic cells suitable for in vivo therapeutic approaches.
The Journal of investigative dermatology - 1 Jan 2000
Müller G, Müller A, Jonuleit H, Steinbrink K, Szalma C, Paragnik L, Lingnau K, Schmidt E, Knop J, Enk A H
Abstract excerpt
UNLABELLED: Standard protocols to generate mouse dendritic cells (DC) generally use culture medium supplemented with fetal calf serum; however, reinjection in vivo of DC cultured in fetal calf serum results in priming to xenogeneic proteins that clearly limits the use of such DC. We therefore established a fetal calf serum-free culture system for the generation of murine DC from bone marrow precursors. DC can be...
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