Article
Isolation and identification of Fur binding genes in Escherichia coli.
Molecules and cells - 31 Oct 1999
Oh M, Chai S H, Wee S
Abstract excerpt
Fur (ferric uptake regulation) binding fragments were isolated by in vitro binding of purified Fur protein with Sau3AI-digested genomic DNA fragments. The Fur-bound DNA fragments were filtered on nitrocellulose paper, isolated, cloned, and sequenced. The protein binding was confirmed by gel retardation assay for five DNA fragments. The sequence data were used to identify the genes by comparison with the GenBank...
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