Article
Identification of Fur regulated genes in the bacterial fish pathogen Photobacterium damselae ssp. piscicida using the Fur titration assay.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine - 1 Dec 2004
Osorio Carlos R, Lemos Manuel L, Braun Volkmar
Abstract excerpt
Bacteria have developed a series of iron-scavenging and transport systems. The expression of many of the iron utilization genes is tightly regulated by the Fe2+ loaded Fur repressor protein. In this study, the Fur titration assay (FURTA) was used to screen for DNA fragments from a genomic DNA library of Photobacterium damselae ssp. piscicida containing potential Fe2+ Fur binding sites or iron binding-proteins...
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