Overproduction of NlpE, a new outer membrane lipoprotein, suppresses the toxicity of periplasmic LacZ by activation of the Cpx signal transduction pathway.

Abstract excerpt

The LamB-LacZ-PhoA tripartite fusion protein is secreted to the periplasm, where it exerts a toxicity of unknown origin during high-level synthesis in the presence of the inducer maltose, a phenotype referred to as maltose sensitivity. We selected multicopy suppressors of this toxicity that allow...

Topics

• Amino Acid Sequence
• Bacterial Outer Membrane Proteins
• Bacterial Proteins
• Base Sequence
• Consensus Sequence
• Enzyme Activation
• Enzyme Repression
• Escherichia coli
• Escherichia coli Proteins
• Gene Expression Regulation, Bacterial
• Heat-Shock Proteins
• Lipoproteins
• Maltose