Article
Characterization of an alg2 mutant of the zygomycete fungus Rhizomucor pusillus.
Glycobiology - 1 Dec 1999
Takeuchi K, Yamazaki H, Shiraishi N, Ohnishi Y, Nishikawa Y, Horinouchi S
Abstract excerpt
The zygomycete fungus Rhizomucor pusillus secretes an aspartic proteinase (MPP) that contains asparagine ( N )-linked oligosaccharides at two sites. Mutant strain 1116 defective in N -glycosylation secretes MPP with truncated oligo-saccharide chains. Lipid-linked oligosaccharides in mutant 1116 were labeled with [6-(3)H]glucosamine and [2-(3)H]mannose, prepared by cycles of solvent extraction, and analyzed by gel...
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