Article
Premature termination codon in the aggrecan gene of nanomelia and its influence on mRNA transport and stability.
Croatian medical journal - 1 Dec 1999
Primorac D, Johnson C V, Lawrence J B, McKinstry M B, Stover M L, Schanfield M S, Andjelinovic S, Tadic T, Rowe D W
Abstract excerpt
AIM: To analyze the influence of the premature termination codon on mRNA transport and stability METHODS: Chondrocyte mRNA was isolated from homozygous and heterozygous nanomelic 17-days old embryos and examined by RT-PCR analysis. To analyze aggrecan mRNA stability, mRNA synthesis was inhibited with DRB [5,6 dichloro-1-(-D-ribofuranosyl benzimidazole)], a specific inhibitor of RNA polymerase II. Visualization of...
Topics
- Aggrecans
- Animals
- Cartilage
- Cartilage Diseases
- Cell Culture Techniques
- Chick Embryo
- Chondrocytes
- Chondroitin Sulfate Proteoglycans
- Codon, Terminator
- Dichlororibofuranosylbenzimidazole
- Extracellular Matrix Proteins
- Genotype
- Lectins, C-Type