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Heterologous Expression and Product Identification of<i>Colletotrichum lagenarium</i>Polyketide Synthase Encoded by the<i>PKS1</i>Gene Involved in Melanin Biosynthesis

1 Jan 1999

Abstract excerpt

The Colletotrichum lagenarium PKS1 gene was expressed in the heterologous fungal host, Aspergillus oryzae, under the starch-inducible alpha-amylase promoter to identify the direct product of polyketide synthase (PKS) encoded by the PKS1 gene. The main compound produced by an A. oryzae transformant was isolated and characterized to be 1,3,6,8-tetrahydroxynaphthalene (T4HN) as its tetraacetate. Since the PKS1 gene...

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PMID: 10501004
DOI: 10.1271/bbb.63.1445

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