Article
Human long-term culture initiating cells are sensitive to benzylguanine and 1,3-bis(2-chloroethyl)-1-nitrosourea and protected after mutant (G156A) methylguanine methyltransferase gene transfer.
Cancer gene therapy - 1 Jan 2000
Koç O N, Reese J S, Szekely E M, Gerson S L
Abstract excerpt
Human hematopoietic progenitors express low levels of O6-alkylguanine-DNA alkyltransferase and are sensitive to 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), particularly following O6-benzylguanine (BG)-mediated O6-alkylguanine-DNA alkyltransferase inhibition. Expression of the BG-resistant mutant (G156A) methylguanine methyltransferase (deltaMGMT) gene in hematopoietic cells confers resistance to BG and BCNU....
Topics
- Antigens, CD34
- Carmustine
- Cell Survival
- Flow Cytometry
- Gene Transfer Techniques
- Guanine
- Hematopoietic Stem Cells
- Humans
- K562 Cells
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase