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Development of genetic methods and construction of a chromosomal glnK1 mutant in Methanosarcina mazei strain Gö1.

Molecular genetics and genomics : MGG - 1 Jun 2005

Ehlers Claudia, Weidenbach Katrin, Veit Katharina, Deppenmeier Uwe, Metcalf William W, Schmitz Ruth A

Abstract excerpt

The methanogenic archaeon Methanosarcina mazei strain Gö1 has so far proven to be genetically intractable due to its low plating efficiency on solid medium and the lack of an effective transformation method. Here, we report the first significant improvement in plating efficiency (up to 10%), which was achieved by (1) selecting for a spontaneous mutant of M. mazei that shows significantly higher resistance to...

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PMID: 15824904
DOI: 10.1007/s00438-005-1128-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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