Article
Continuous culture of Methanococcus maripaludis under defined nutrient conditions.
FEMS microbiology letters - 1 Sept 2004
Haydock Andrew K, Porat Iris, Whitman William B, Leigh John A
Abstract excerpt
To study global regulation in the methanogenic archaeon Methanococcus maripaludis, we devised a system for steady-state growth in chemostats. New Brunswick Bioflo 110 bioreactors were equipped with controlled delivery of hydrogen, nitrogen, carbon dioxide, hydrogen sulfide, and anaerobic medium. We determined conditions and media compositions for growth with three different limiting nutrients, hydrogen,...
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