Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An in vivo library-versus-library selection of optimized protein-protein interactions.

Nature biotechnology - 1 Jul 1999

Pelletier J N, Arndt K M, Plückthun A, Michnick S W

Abstract excerpt

We describe a rapid and efficient in vivo library-versus-library screening strategy for identifying optimally interacting pairs of heterodimerizing polypeptides. Two leucine zipper libraries, semi-randomized at the positions adjacent to the hydrophobic core, were genetically fused to either one of two designed fragments of the enzyme murine dihydrofolate reductase (mDHFR), and cotransformed into Escherichia coli....

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Dimerization
Share to Leucine Zippers
Share to Peptide Fragments

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Peptide Library

Share to Protein Folding

Share to Protein Structure, Secondary

Share to Recombinant Fusion Proteins

Share to Tetrahydrofolate Dehydrogenase

Source

Identifiers

PMID: 10404162
DOI: 10.1038/10897

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An in vivo library-versus-library selection of optimized protein-protein interactions.PMID 10404162Read ↗

Statistical Coupling Analysis-Guided Library Design for the Discovery of Mutant Luciferases.Semantic proximity Read ↗

Discovery of improved EGF agonists using a novel in vitro screening platform.Semantic proximity Read ↗

[Toward creation of proteins by combination of block shuffling and in vitro selection methods].Semantic proximity Read ↗

Versatile selection technology for intracellular protein-protein interactions mediated by a unique bacterial hitchhiker transport mechanism.Semantic proximity Read ↗

A high throughput approach for the generation of orthogonally interacting protein pairs.Semantic proximity Read ↗

Synthesis of functional proteins by mixing peptide motifs.Semantic proximity Read ↗

Controlled assembly of bifunctional chimeric protein cages and composition analysis using noncovalent mass spectrometry.Semantic proximity Read ↗

Construction and maintenance of randomized retroviral expression libraries for transmembrane protein engineering.Semantic proximity Read ↗

Construction and characterization of a single polypeptide chain containing two enzymatically active dihydrofolate reductase domains.Semantic proximity Read ↗

Library-on-Library Intercellular Labeling for Selection of Biotin Ligase and Acceptor Peptides.Semantic proximity Read ↗

DNA display of fragment pairs as a tool for the discovery of novel biologically active small moleculesSemantic proximity Read ↗

A novel approach to selection of functional proteins.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.