Article
Mobilisation of the streptococcal plasmid pMV158: interactions of MobM protein with its cognate oriT DNA region.
Molecular & general genetics : MGG - 1 Jun 1999
Grohmann E, Guzmán L M, Espinosa M
Abstract excerpt
The streptococcal plasmid pMV158 encodes the relaxase protein, MobM, involved in its mobilisation. Purified MobM protein specifically cleaved supercoiled or single-stranded DNA containing the plasmid origin of transfer, oriT. Gel retardation and DNase I footprinting assays performed with DNA fragments containing the plasmid oriT provided evidence for specific binding of MobM by oriT DNA. Dissection of the...
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