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Article

A new high-throughput AFLP approach for identification of new genetic polymorphism in the genome of the clonal microorganism Mycobacterium tuberculosis.

Journal of microbiological methods - 1 Jan 2004

van den Braak Nicole, Simons Guus, Gorkink Roy, Reijans Martin, Eadie Kimberly, Kremers Kristin, van Soolingen Dick, Savelkoul Paul, Verbrugh Henri, van Belkum Alex

Abstract excerpt

We have here applied high-throughput amplified fragment length polymorphism (htAFLP) analysis to strains belonging to the five classical species of the Mycobacterium tuberculosis complex. Using 20 strains, three enzyme combinations and eight selective amplification primer pairs, 24 AFLP reactions were performed per strain. Overall, this resulted in 480 DNA fingerprints and more than 1200 htAFLP-amplified PCR...

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Source

Identifiers

PMID: 14706750
DOI: 10.1016/j.mimet.2003.09.018

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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