Article
A KP element inserted between the two promoters of the alcohol dehydrogenase gene of Drosophila melanogaster differentially affects expression in larvae and adults.
Biochemical genetics - 1 Dec 1998
Wu Y H, Wilks A V, Gibson J B
Abstract excerpt
An allele of the Drosophila melanogaster alcohol dehydrogenase (Adh) gene has a 1.15-kb KP element inserted, in the same orientation as Adh transcription, five nucleotides upstream of the distal transcription start site. The target site--GTCCAAGT--in Adh between nucleotides -13 and -6 is duplicated at both ends of the insertion. Adult flies with either one or two copies of the allele have less than 12% of the...
Topics
- Alcohol Dehydrogenase
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- DNA Transposable Elements
- Drosophila melanogaster
- Gene Expression Regulation, Developmental
- Gene Expression Regulation, Enzymologic
- Heterozygote
- Larva
- Phenotype
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- Sequence Deletion
- Transcription, Genetic