Article
Aberrant splicing of a naturally occurring alcohol dehydrogenase null activity allele in Drosophila melanogaster.
Genome - 1 Dec 1990
Freeth A L, Gibson J B, Wilks A V
Abstract excerpt
The DNA sequence of a naturally occurring alcohol dehydrogenase null activity allele, AdhnAC14, has eight extra nucleotides (in two groups of four) in the second intron, commencing six bases 3' from the 5' splice site. A stop codon was also found in exon 2. S1 nuclease protection experiments have shown that the insertions in intron 2 disrupt the correct splicing of intron 2. The null allele produces a transcript...
Topics
- Alcohol Dehydrogenase
- Alleles
- Animals
- Base Sequence
- Drosophila melanogaster
- Introns
- Molecular Sequence Data
- RNA Splicing
- Restriction Mapping