Heptameric structures of two alpha-hemolysin mutants imaged with in situ atomic force microscopy.

doi:10.1002/(SICI)1097-0029(19990301)44:5<353::AID-JEMT6>3.0.CO;2-0

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Heptameric structures of two alpha-hemolysin mutants imaged with in situ atomic force microscopy.

Microscopy research and technique - 1 Mar 1999

Malghani M S, Fang Y, Cheley S, Bayley H, Yang J

Abstract excerpt

Atomic force microscopy has been used to study self-assembled structures of two alpha-hemolysin mutants. For a mutant (alphaHL-H5) that was locked into the prepore state on fluid phase egg-PC membranes, we visualized, for the first time, heptameric prepores and showed that the 7-fold axis in the prepore lies perpendicular to the membrane surface. For another mutant (TCM) with the transmembrane domain, the...

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Heptameric structures of two alpha-hemolysin mutants imaged with in situ atomic force microscopy.

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