Article
Novel technique for the direct flow cytofluorometric analysis of human basophils in unseparated blood and bone marrow, and the characterization of phenotype and peroxidase of human basophils.
Cytometry - 1 Mar 1999
Toba K, Koike T, Shibata A, Hashimoto S, Takahashi M, Masuko M, Azegami T, Takahashi H, Aizawa Y
Abstract excerpt
BACKGROUND: No technique has been reported to analyze directly the antigen expression on basophil leukocytes when using a flow cytometer; therefore, the exact phenotype of human basophils and the character of the peroxidase in basophils are not well understood. METHODS: Human blood basophils were purified by using an antibody against high-affinity Fc epsilon receptor (hFcepsilonR) and a MACS magnetic cell sorting...
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