Article
[Using flow cytometry to detect peripheral blood eosinophils and their related molecules].
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 1 Jul 2004
Cao Yun-xin, Shi Jie-ran, Han Wei-ning
Abstract excerpt
AIM: To set up an accurate and rapid method to detect the phenotype of peripheral blood eosinophils using flow cytometry. METHODS: 10(-4) mol/L of theophylline, 10(-4) of dexamethasone and 10(-8) mol/L of rhIL-5 were added to peripheral blood sampled from normal human subjects. Anti-CD16-PE mAb and FITC-labelled mAbs to eosinophil's molecules were used to perform double labeling staining. Simultaneously, CD16-FL2...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.