Article
Mitochondrial copper metabolism in yeast: mutational analysis of Sco1p involved in the biogenesis of cytochrome c oxidase.
Current genetics - 1 Mar 1999
Rentzsch A, Krummeck-Weiss G, Hofer A, Bartuschka A, Ostermann K, Rödel G
Abstract excerpt
Saccharomyces cerevisiae Sco1p is believed to be involved in the transfer of copper from the carrier Cox17p to the mitochondrial cytochrome c oxidase subunits 1 and 2. We here report on the results of a mutational analysis of Sco1p. The two cysteine residues of a potential metal-binding motif (CxxxC) are essential for protein function as shown by their substitution by alanines. Chimeras consisting of Sco1p and...
Topics
- Amino Acid Sequence
- Binding Sites
- Copper
- Electron Transport Complex IV
- Fungal Proteins
- Membrane Proteins
- Mitochondria
- Mitochondrial Proteins
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins