Article
Membrane deinsertion of SecA underlying proton motive force-dependent stimulation of protein translocation.
The EMBO journal - 15 Feb 1999
Nishiyama K, Fukuda A, Morita K, Tokuda H
Abstract excerpt
The proton motive force (PMF) renders protein translocation across the Escherichia coli membrane highly efficient, although the underlying mechanism has not been clarified. The membrane insertion and deinsertion of SecA coupled to ATP binding and hydrolysis, respectively, are thought to drive the translocation. We report here that PMF significantly decreases the level of membrane-inserted SecA. The prlA4 mutation...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Adenylyl Imidodiphosphate
- Bacterial Outer Membrane Proteins
- Bacterial Proteins
- Biological Transport
- Cell Membrane
- Endopeptidase K
- Escherichia coli
- Escherichia coli Proteins
- Kinetics
- Membrane Transport Proteins
- Mutation
- Protein Precursors