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Article

Generation of Retroviral Vector for Clinical Studies Using Transient Transfection

1 Jan 1999

Abstract excerpt

Transient transfection of 293T cells was utilized to produce high-titer murine recombinant retroviral vectors for clinical studies. This system was initially optimized by gene transfer using different retroviral envelope proteins into activated human CD4+ T lymphocytes in vitro. Higher titer and infectivity were obtained than with stable murine producer lines; titers of 0.3-1 x 10(7) infectious units per...

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Identifiers

PMID: 10022537
DOI: 10.1089/10430349950019255

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